Users interested in trying out these technologies are highly welcome! Fast 3D acquisitions are covered by spinning disc and light-sheet systems.ĬARS, QPI and SIM technologies, offered by Prague Node, are currently undertaking a Proof-of-Concept Study (PCS) to become official EuBI technologies.
Plants growing in their natural gravity conditions can be directly visualized on the vertical microscope stage of CZ LSM880 with Airyscan.
Innovative low-toxicity label-free imaging is offered by quantitative phase imaging (QPI). Label-free imaging profits from non-linear processes induced by femtosecond NIR lasers and offers method like SHG, THG, autofluorescence FLIM including metabolic imaging and CARS for lipid droplets visualization. Information about molecular dynamics and interactions can be obtained by functional imaging, particularly from spatial-temporal correlation analysis (point, line and image F(C)CS), FRAP, photoactivation, FRET, FLIM or PLIM. In super-resolution imaging all main approaches are covered, including STED, SIM and SMLM, by multiple commercial systems from different manufacturers. The main strength is the wide and deep expertise covering most of light and electron microscopy biological applications, allowed by the complementary nature of collaborating core facilities and more than 25 FTE of expert staff operating more than 45 advanced microscopy systems. Broad expertise in experiment design and data acquisition is also complemented by extensive data analysis services. In the area of electron microscopy, the Node provides expertise and cutting edge equipment for a broad range of biological sample preparation and ultrastructural imaging techniques, including some cryo techniques, electron tomography, SBF-SEM, FIB-SEM and CLEM. Light microscopy instrumentation includes microscopy systems that range from multi-functional point and spinning disc confocal microscopes, light-sheet system up to high-end multi-photon and super-resolution microscopy systems.
The Node is also active in organizing training and courses focused on theoretical and practical aspects of basic and advanced microscopy techniques. The Czech Advanced Light and Electron Microscopy Multi Modal Multi Sited Node located in Prague and in České Budějovice offers a wide range of state-of-the-art light and electron microscopy equipment and techniques, offered by five closely collaborating core facilities. CZECH REPUBLIC Advanced Light And Electron Microscopy Node Prague CZ